Journal: ACS chemical neuroscience

Article Title: Passage Variation of PC12 Cells Results in Inconsistent Susceptibility to Externally Induced Apoptosis

doi: 10.1021/acschemneuro.6b00208

Figure Lengend Snippet: Optimization and validation of etoposide-induced cell death in PC12 cells (passage 9–13). 50 × 103 cells/well. (a) Approximately 50% cell death was observed after 48 h. Four replicates per experiment. Values are represented as mean ± SD. Multiple t test, Holm-Sidak method, alpha = 5.000%, ****p < 0.0001. (b) Fluorescence measured after 48 h. Flupirtine at 3 μM concentration rescued the cells from apoptosis. Experiment repeated thrice. Values are represented as mean ± SD. Unpaired two-tailed t test, 95% CI, ****p < 0.0001 in comparison to vehicle. ####p < 0.0001 in comparison to etoposide treatment.

Article Snippet: 5 Etoposide (Chem-Impex International, 28435) was stored as a working stock solution of 150 μ g/mL at −20 °C for up to 3 months.

Techniques: Fluorescence, Concentration Assay, Two Tailed Test, Comparison

Journal: ACS chemical neuroscience

Article Title: Passage Variation of PC12 Cells Results in Inconsistent Susceptibility to Externally Induced Apoptosis

doi: 10.1021/acschemneuro.6b00208

Figure Lengend Snippet: NGF differentiated PC12 cells show a statistically significant difference in sensitivity to apoptosis induced by etoposide between early and late passages at 72 h post insult. (a) Passage 10. Three replicates per experiment. Values are represented as mean ± SD. One-way ANOVA, Dunnett test, 95% CI. ****p < 0.0001. (b) Passage 17. Three replicates per experiment. Values are represented as mean ± SD. One-way ANOVA, Dunnett test, 95% CI. ****p < 0.0001. (c) Comparison of 72 h etoposide treatment in passage 10 and 17 PC12 cells differentiated using NGF. Unpaired two-tailed t test, 95% CI, ****p < 0.0001.

Article Snippet: 5 Etoposide (Chem-Impex International, 28435) was stored as a working stock solution of 150 μ g/mL at −20 °C for up to 3 months.

Techniques: Comparison, Two Tailed Test

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Distinct endotypes of steroid-resistant asthma characterized by IL-17A high and IFN-γ high immunophenotypes: Potential benefits of calcitriol

doi: 10.1016/j.jaci.2015.01.026

Figure Lengend Snippet: Clinical trial schematic. Patients with severe asthma were recruited and given prednisolone at screening visit 1 and returned 2 weeks later at screening visit 2 when patients with SS asthma were excluded from the trial and patients with SR asthma were retained. After a 4-week washout period, the patients with SR asthma were randomly assigned to receive calcitriol or placebo for 1 month (treatment visit 1). After 2 weeks (treatment visit 2), the patients were started on additional oral prednisolone and then returned 2 weeks later for the final visit (treatment visit 3). Lung function was assessed at all clinical trial visits except treatment visit 1. CD8-depleted PBMCs were assessed from peripheral blood collected at screening visit 1 and treatment visit 3.

Article Snippet: Steroid resistance was defined as less than 10% improvement in baseline prebronchodilator FEV 1 after a 14-day course of oral prednisolone (40 mg/1.73 m 2 /d; Wockhardt UK, Wrexham, United Kingdom) in eligible patients.

Techniques:

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Distinct endotypes of steroid-resistant asthma characterized by IL-17A high and IFN-γ high immunophenotypes: Potential benefits of calcitriol

doi: 10.1016/j.jaci.2015.01.026

Figure Lengend Snippet: Peripheral leukocyte counts. A, Peripheral blood total and differential leukocyte counts in the patients with SS asthma (solid symbols) and patients with SR asthma (open symbols) before and after 2 weeks of prednisolone. B and C, Total B cells (CD19 + ), T cells (CD3 + ), and CD4 + and CD8 + T cells were assessed by using ex vivo flow cytometric analysis of peripheral blood from screening visit 1 of both patients with SS and those with SR asthma (Fig E2, B ), as well as in the same patients with SR asthma separated based on their assignment to calcitriol or placebo treatment (Fig E2, C ). Data were assessed by using the Wilcoxon matched-pairs statistical test (Fig E2, A ) or the Mann-Whitney U statistical test (Fig E2, B and C ). * P ≤ .05, ** P < .01, and *** P < .001.

Article Snippet: Steroid resistance was defined as less than 10% improvement in baseline prebronchodilator FEV 1 after a 14-day course of oral prednisolone (40 mg/1.73 m 2 /d; Wockhardt UK, Wrexham, United Kingdom) in eligible patients.

Techniques: Ex Vivo, MANN-WHITNEY

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Distinct endotypes of steroid-resistant asthma characterized by IL-17A high and IFN-γ high immunophenotypes: Potential benefits of calcitriol

doi: 10.1016/j.jaci.2015.01.026

Figure Lengend Snippet: IL-17A is negatively and IL-13 is positively associated with clinical response to 2 weeks of prednisolone. Cytokine production (A) and ratio of IL-13/IL-17A and IL-10/IL-17A cytokine production (B) separated based on change in absolute lung function in response to 2 weeks of prednisolone (ΔFEV 1 ) are shown. Data are means and 95% CIs and assessed by using Kruskal-Wallis 1-way ANOVA with the Dunn multiple comparison post hoc statistical test. * P ≤ .05 and ** P < .01.

Article Snippet: Steroid resistance was defined as less than 10% improvement in baseline prebronchodilator FEV 1 after a 14-day course of oral prednisolone (40 mg/1.73 m 2 /d; Wockhardt UK, Wrexham, United Kingdom) in eligible patients.

Techniques: Comparison

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Distinct endotypes of steroid-resistant asthma characterized by IL-17A high and IFN-γ high immunophenotypes: Potential benefits of calcitriol

doi: 10.1016/j.jaci.2015.01.026

Figure Lengend Snippet: IL-17A and IFN-γ production predict glucocorticoid responsiveness. A, Comparison of IL-17A and IFN-γ production in patients with SS asthma (solid circles) and patients with SR asthma (open squares) . B, Combined production of IL-17A and IFN-γ correlated with change in absolute lung function (ΔFEV 1 ) in response to 2 weeks of prednisolone, as assessed by using the Spearman rank correlation statistical test. C, ROC curves for prediction of steroid resistance based on production of IFN-γ, IL-17A, and their combination.

Article Snippet: Steroid resistance was defined as less than 10% improvement in baseline prebronchodilator FEV 1 after a 14-day course of oral prednisolone (40 mg/1.73 m 2 /d; Wockhardt UK, Wrexham, United Kingdom) in eligible patients.

Techniques: Comparison

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Distinct endotypes of steroid-resistant asthma characterized by IL-17A high and IFN-γ high immunophenotypes: Potential benefits of calcitriol

doi: 10.1016/j.jaci.2015.01.026

Figure Lengend Snippet: Calcitriol treatment decreases expression of IL-17A in cultures from patients with SR asthma. Cytokine production by CD8-depleted PBMCs from patients with SR asthma in the presence of dexamethasone (10 −x mol/L) in vitro after 4 weeks of calcitriol or placebo therapy and 2 weeks of oral prednisolone therapy and cells stimulated with CD3 plus IL-2 (A) and additional IL-4 (B) is shown. Open triangles , Calcitriol; solid triangles , placebo. Data are means and 95% CIs. Data were assessed by using 2-way ANOVA with Dunnett multiple comparisons tests. * P ≤ .05, ** P < .01, and *** P < .001.

Article Snippet: Steroid resistance was defined as less than 10% improvement in baseline prebronchodilator FEV 1 after a 14-day course of oral prednisolone (40 mg/1.73 m 2 /d; Wockhardt UK, Wrexham, United Kingdom) in eligible patients.

Techniques: Expressing, In Vitro

Journal: The Journal of Allergy and Clinical Immunology

Article Title: Distinct endotypes of steroid-resistant asthma characterized by IL-17A high and IFN-γ high immunophenotypes: Potential benefits of calcitriol

doi: 10.1016/j.jaci.2015.01.026

Figure Lengend Snippet: Effects of calcitriol on lung function and IL-13 and IL-17A levels. Cytokine production was separated based on individual changes in absolute lung function (ΔFEV 1 ) in response to 2 weeks of prednisolone before (baseline; black bars ) and after (after 4 weeks of calcitriol and 2 weeks of prednisolone; white bars ) treatment. Numbers of patients with SR asthma are indicated in parentheses above.

Article Snippet: Steroid resistance was defined as less than 10% improvement in baseline prebronchodilator FEV 1 after a 14-day course of oral prednisolone (40 mg/1.73 m 2 /d; Wockhardt UK, Wrexham, United Kingdom) in eligible patients.

Techniques: